Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Arachis hypogaea |
Protein Variants | Comment | Organism |
---|---|---|
K160R | site-directed mutagenesis, inactive mutant enzyme | Arachis hypogaea |
Y148F | site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme | Arachis hypogaea |
Y213F | site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme | Arachis hypogaea |
Y297F | site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme | Arachis hypogaea |
Y317F | site-directed mutagenesis, the mutant enzyme shows altered inhibition kinetics with tyrosine kinase inhibitors compared to the wild-type enzyme | Arachis hypogaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
genistein | competitive inhibition versus ATP, uncompetitive inhibition versus histone H1 | Arachis hypogaea | |
additional information | molecular docking with tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes | Arachis hypogaea | |
staurosporine | competitive versus ATP, mixed inhibition versus histone H1 | Arachis hypogaea | |
tyrphostin | competitive versus ATP, mixed inhibition versus histone H1 | Arachis hypogaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arachis hypogaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Arachis hypogaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
recombinant His-tagged wild-type enzyme, gel filtration | Arachis hypogaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arachis hypogaea | the enzyme is developmentally regulated | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arachis hypogaea | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs autophosphorylation, mechanism and phosphorylation sites, overview | Arachis hypogaea |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arachis hypogaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone H1 | - |
Arachis hypogaea | ADP + phosphorylated histone H1 | - |
? | |
additional information | the enzyme is developmentally regulated | Arachis hypogaea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling of wild-type and mutant enzymes based on the X-ray structure of homologous sequences, computational docking and molecular dynamics simulations, overview | Arachis hypogaea |
Synonyms | Comment | Organism |
---|---|---|
serine/threonine/tyrosine protein kinase | - |
Arachis hypogaea |
STY protein kinase | - |
Arachis hypogaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arachis hypogaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arachis hypogaea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arachis hypogaea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics of tyrosine kinase inhibitors, recombinant His-tagged wild-type and mutant enzymes | Arachis hypogaea | |
0.000014 | - |
tyrphostin | versus ATP, pH 7.5, 30°C, recombinant mutant Y213F | Arachis hypogaea | |
0.000017 | - |
genistein | versus ATP, pH 7.5, 30°C, recombinant mutant Y213F | Arachis hypogaea | |
0.000018 | - |
tyrphostin | versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.00002 | - |
genistein | versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.000055 | - |
tyrphostin | versus ATP, pH 7.5, 30°C, recombinant mutant Y317F | Arachis hypogaea | |
0.000056 | - |
staurosporine | pH 7.5, 30°C, recombinant mutant Y213F | Arachis hypogaea | |
0.000059 | - |
genistein | versus ATP, pH 7.5, 30°C, recombinant mutant Y317F | Arachis hypogaea | |
0.000069 | - |
staurosporine | versus ATP, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.000112 | - |
staurosporine | pH 7.5, 30°C, recombinant mutant Y317F | Arachis hypogaea | |
0.000186 | - |
tyrphostin | versus ATP, pH 7.5, 30°C, recombinant mutant Y297F | Arachis hypogaea | |
0.000187 | - |
tyrphostin | versus ATP, pH 7.5, 30°C, recombinant mutant Y148F | Arachis hypogaea | |
0.000191 | - |
genistein | versus ATP, pH 7.5, 30°C, recombinant mutant Y148F | Arachis hypogaea | |
0.000194 | - |
genistein | versus ATP, pH 7.5, 30°C, recombinant mutant Y297F | Arachis hypogaea | |
0.000202 | - |
tyrphostin | versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.000426 | - |
genistein | versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.000455 | - |
staurosporine | versus histone H1, pH 7.5, 30°C, recombinant wild-type enzyme | Arachis hypogaea | |
0.000464 | - |
staurosporine | pH 7.5, 30°C, recombinant mutant Y297F | Arachis hypogaea | |
0.000549 | - |
staurosporine | versus histone H1, pH 7.5, 30°C, recombinant mutant Y213F | Arachis hypogaea | |
0.000571 | - |
staurosporine | pH 7.5, 30°C, recombinant mutant Y148F | Arachis hypogaea | |
0.000599 | - |
staurosporine | versus histone H1, pH 7.5, 30°C, recombinant mutant Y317F | Arachis hypogaea | |
0.000621 | - |
staurosporine | versus histone H1, pH 7.5, 30°C, recombinant mutant Y148F | Arachis hypogaea | |
0.000694 | - |
staurosporine | versus histone H1, pH 7.5, 30°C, recombinant mutant Y297F | Arachis hypogaea |